MEDICAL MICROSCOPY

STED microscopy: increased resolution for medical research?

Optical imaging is crucial for addressing fundamental problems in all areas of life science. With the use of confocal and two-photon fluorescence microscopy, complex dynamic structures and functions in a plethora of tissue and cell types have been visualized. However, the resolution of 'classical' optical imaging methods is poor due to the diffraction limit and does not allow resolution of the ...

Virtual Microscopy in Medical Images: a Survey

A major objective of the present survey is to provide a comprehensive vision of the most recent methods for the construction of a Virtual Microscopy Viewer (VMV). The Virtual Microscopy (VM) is the microscopy area that provides a realistic digital emulation of a conventional light microscope and the VMV is the software tool that provides such emulation. Construction of a mega-image by stitching...

Innovations in medical imaging and virtual microscopy.

Imaging with Rays: Microscopy, Medical Imaging, and Computer Vision

In this paper we broadly consider techniques which utilize projections on rays for data collection, with particular emphasis on optical techniques. We formulate a variety of imaging techniques as either special cases or extensions of tomographic reconstruction. We then consider how the techniques must be extended to describe objects containing occlusion, as with a self-occluding opaque object. ...

Evaluation of virtual microscopy in medical histology teaching.

Histology stands as a major discipline in the life science curricula, and the practice of teaching it is based on theoretical didactic strategies along with practical training. Traditionally, students achieve practical competence in this subject by learning optical microscopy. Today, students can use newer information and communication technologies in the study of digital microscopic images. A ...